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Registro Completo |
Biblioteca(s): |
Embrapa Algodão. |
Data corrente: |
22/11/2005 |
Data da última atualização: |
22/11/2005 |
Autoria: |
EVANGELISTA-RODRIGUES, A.; SILVA, E. M. S. da; BESERRA, E. M. F.; RODRIGUES, M. L. |
Título: |
Análise físico-química dos méis das abelhas Apis mellifera e Melipona scutellaris produzidos em duas regiões no Estado da Paraíba. |
Ano de publicação: |
2005 |
Fonte/Imprenta: |
Ciência Rural , v.35, n.1, p.1166-1171, 2005. |
Idioma: |
Português |
Palavras-Chave: |
Abelha sem ferrão; Brasil; Paraíba; Produtor apícola. |
Thesagro: |
Apicultura. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00646naa a2200205 a 4500 001 1274144 005 2005-11-22 008 2005 bl uuuu u00u1 u #d 100 1 $aEVANGELISTA-RODRIGUES, A. 245 $aAnálise físico-química dos méis das abelhas Apis mellifera e Melipona scutellaris produzidos em duas regiões no Estado da Paraíba. 260 $c2005 650 $aApicultura 653 $aAbelha sem ferrão 653 $aBrasil 653 $aParaíba 653 $aProdutor apícola 700 1 $aSILVA, E. M. S. da 700 1 $aBESERRA, E. M. F. 700 1 $aRODRIGUES, M. L. 773 $tCiência Rural$gv.35, n.1, p.1166-1171, 2005.
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Embrapa Algodão (CNPA) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
16/11/2017 |
Data da última atualização: |
23/11/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
RAMOS-GONZÁLEZ, P. L.; CHABI-JESUS, C.; GUERRA-PERAZA, O.; TASSI, A. D.; KITAJIMA, E. W.; HARAKAVA, R.; SALAROLI, R. B.; ASTUA, J. de F. |
Afiliação: |
PEDRO LUIS RAMOS-GONZÁLEZ; CAMILA CHABI-JESUS; ORLENE GUERRA-PERAZA; ALINE DANIELE TASSI; ELLIOT WATANABE KITAJIMA; RICARDO HARAKAVA; RENATO BARBOSA SALAROLI; JULIANA DE FREITAS ASTUA, CNPMF. |
Título: |
Citrus leprosis virus N: a new dichorhavirus causing citrus leprosis disease. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Phytopathology, v.107, n.8, August 2017. |
ISSN: |
0031-949X |
DOI: |
10.1094/PHYTO-02-17-0042-R |
Idioma: |
Inglês |
Conteúdo: |
Citrus leprosis (CL) is a viral disease endemic to the Western Hemisphere that produces local necrotic and chlorotic lesions on leaves, branches, and fruit and causes serious yield reduction in citrus orchards. Samples of sweet orange (Citrus × sinensis) trees showing CL symptoms were collected during a survey in noncommercial citrus areas in the southeast region of Brazil in 2013 to 2016. Transmission electron microscopy analyses of foliar lesions confirmed the presence of rod-like viral particles commonly associated with CL in the nucleus and cytoplasm of infected cells. However, every attempt to identify these particles by reverse-transcription polymerase chain reaction tests failed, even though all described primers for the detection of known CL-causing cileviruses and dichorhaviruses were used. Next-generation sequencing of total RNA extracts from three symptomatic samples revealed the genome of distinct, although highly related (>92% nucleotide sequence identity), viruses whose genetic organization is similar to that of dichorhaviruses. The genome sequence of these viruses showed <62% nucleotide sequence identity with those of orchid fleck virus and coffee ringspot virus. Globally, the deduced amino acid sequences of the open reading frames they encode share 32.7 to 63.8% identity with the proteins of the dichorhavirids. Mites collected from both the naturally infected citrus trees and those used for the transmission of one of the characterized isolates to Arabidopsis plants were anatomically recognized as Brevipalpus phoenicis sensu stricto. Molecular and biological features indicate that the identified viruses belong to a new species of CL-associated dichorhavirus, which we propose to call Citrus leprosis N dichorhavirus. Our results, while emphasizing the increasing diversity of viruses causing CL disease, lead to a reevaluation of the nomenclature of those viruses assigned to the genus Dichorhavirus. In this regard, a comprehensive discussion is presented. MenosCitrus leprosis (CL) is a viral disease endemic to the Western Hemisphere that produces local necrotic and chlorotic lesions on leaves, branches, and fruit and causes serious yield reduction in citrus orchards. Samples of sweet orange (Citrus × sinensis) trees showing CL symptoms were collected during a survey in noncommercial citrus areas in the southeast region of Brazil in 2013 to 2016. Transmission electron microscopy analyses of foliar lesions confirmed the presence of rod-like viral particles commonly associated with CL in the nucleus and cytoplasm of infected cells. However, every attempt to identify these particles by reverse-transcription polymerase chain reaction tests failed, even though all described primers for the detection of known CL-causing cileviruses and dichorhaviruses were used. Next-generation sequencing of total RNA extracts from three symptomatic samples revealed the genome of distinct, although highly related (>92% nucleotide sequence identity), viruses whose genetic organization is similar to that of dichorhaviruses. The genome sequence of these viruses showed <62% nucleotide sequence identity with those of orchid fleck virus and coffee ringspot virus. Globally, the deduced amino acid sequences of the open reading frames they encode share 32.7 to 63.8% identity with the proteins of the dichorhavirids. Mites collected from both the naturally infected citrus trees and those used for the transmission of one of the characterized isolates to Arabidopsis ... Mostrar Tudo |
Palavras-Chave: |
Plant disease. |
Thesagro: |
Doença de planta; Fruta cítrica. |
Thesaurus NAL: |
Citrus. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02790naa a2200277 a 4500 001 2079883 005 2017-11-23 008 2017 bl --- 0-- u #d 022 $a0031-949X 024 7 $a10.1094/PHYTO-02-17-0042-R$2DOI 100 1 $aRAMOS-GONZÁLEZ, P. L. 245 $aCitrus leprosis virus N$ba new dichorhavirus causing citrus leprosis disease.$h[electronic resource] 260 $c2017 520 $aCitrus leprosis (CL) is a viral disease endemic to the Western Hemisphere that produces local necrotic and chlorotic lesions on leaves, branches, and fruit and causes serious yield reduction in citrus orchards. Samples of sweet orange (Citrus × sinensis) trees showing CL symptoms were collected during a survey in noncommercial citrus areas in the southeast region of Brazil in 2013 to 2016. Transmission electron microscopy analyses of foliar lesions confirmed the presence of rod-like viral particles commonly associated with CL in the nucleus and cytoplasm of infected cells. However, every attempt to identify these particles by reverse-transcription polymerase chain reaction tests failed, even though all described primers for the detection of known CL-causing cileviruses and dichorhaviruses were used. Next-generation sequencing of total RNA extracts from three symptomatic samples revealed the genome of distinct, although highly related (>92% nucleotide sequence identity), viruses whose genetic organization is similar to that of dichorhaviruses. The genome sequence of these viruses showed <62% nucleotide sequence identity with those of orchid fleck virus and coffee ringspot virus. Globally, the deduced amino acid sequences of the open reading frames they encode share 32.7 to 63.8% identity with the proteins of the dichorhavirids. Mites collected from both the naturally infected citrus trees and those used for the transmission of one of the characterized isolates to Arabidopsis plants were anatomically recognized as Brevipalpus phoenicis sensu stricto. Molecular and biological features indicate that the identified viruses belong to a new species of CL-associated dichorhavirus, which we propose to call Citrus leprosis N dichorhavirus. Our results, while emphasizing the increasing diversity of viruses causing CL disease, lead to a reevaluation of the nomenclature of those viruses assigned to the genus Dichorhavirus. In this regard, a comprehensive discussion is presented. 650 $aCitrus 650 $aDoença de planta 650 $aFruta cítrica 653 $aPlant disease 700 1 $aCHABI-JESUS, C. 700 1 $aGUERRA-PERAZA, O. 700 1 $aTASSI, A. D. 700 1 $aKITAJIMA, E. W. 700 1 $aHARAKAVA, R. 700 1 $aSALAROLI, R. B. 700 1 $aASTUA, J. de F. 773 $tPhytopathology$gv.107, n.8, August 2017.
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